Bioinformatical Analysis of PHB Depolymerases from the Phototrophic Bacterium Rhodopseudomonas Palustris Using Computational Tools
Ramchander Merugu
University College of Science and Informatics, Mahatma Gandhi University, Nalgonda, India
Sireesha Radarapu
University College of Science and Informatics, Mahatma Gandhi University, Nalgonda, India
D. Jayasree
Bojjam Narasimhulu Pharmacy College for Women, JNTU, Hyderabad, India
Kalyani Sambaru
University College of Science and Informatics, Mahatma Gandhi University, Nalgonda, India
Shivaranjani Manda
University College of Science, Saifabad, Osmania University, Hyderabad,India
Keywords: PHB, Depolymerases, Rhodopseudomonas palustris, Secondary structure.
Abstract
In this study, PHB depolymerases from seven bacterial Rhodopseudomonas palustris were analyzed and presented in this communication. The composition of alanine, leucine and valine were the highest while lowest concentrations of asparagine and lysine residues were seen when compared to other aminoacids. pI value of Rp3 was 10.47 while the lowest pI of 5.67 was seen in Rp1. The instability index of all the depolymerases varied while for most of them it was less than 40 showing that some of them are stable while others are unstable. Aliphatic index was found to span within a range of 104 to 121. Secondary structural analysis of the depolymerases showed the pre-dominance of α-helices followed by random coils for all the depolymerases except Rp1 depolymerase. Significance of the above results are discussed in the light of existing literature.