The potential of specific profilin based fingerprinting differs in legume species

Abstract

One requirement for using germplasm in agricultural development initiatives is its characterization, which necessitates knowledge of the genetic polymorphism and relationships among the individual varieties. Up to now, different DNA markers were utilized for this purposed, one of the newest are those for coding regions. Here, we aimed to investigate polymorphism and genetic relationships among 24 varieties of Cicer arietinum L. and 23 varieties of Pisum sativum L. using profilin based fingerprinting. PCR approach was used to generate homologue amplicons of plant profilins and UPGMA grouping for visualization of obtained fingerprint similarity. Amplification results showed different results for analysed legume species, where the higher polymorphism at the level of 96% was obtained within the accessions of pea varieties, as for chickpea fingerprints a very similar profiles were generated with only a limited amplicons of a total of three different length of 150 bp, 182 bp and 348 bp.  This information could be useful in breeding strategies for the improvement of chickpea and pea accessions.